Data Paper |
Corresponding author: Stanislas Talaga ( stantalaga@free.fr ) Academic editor: Gunnar Kvifte
© 2015 Stanislas Talaga, Jérôme Murienne, Alain Dejean, Céline Leroy.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Talaga S, Murienne J, Dejean A, Leroy C (2015) Online database for mosquito (Diptera, Culicidae) occurrence records in French Guiana. ZooKeys 532: 107-115. https://doi.org/10.3897/zookeys.532.6176
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A database providing information on mosquito specimens (Arthropoda: Diptera: Culicidae) collected in French Guiana is presented. Field collections were initiated in 2013 under the auspices of the CEnter for the study of Biodiversity in Amazonia (CEBA: http://www.labexceba.fr/en/). This study is part of an ongoing process aiming to understand the distribution of mosquitoes, including vector species, across French Guiana. Occurrences are recorded after each collecting trip in a database managed by the laboratory
Occurrence, French Guiana, Neotropics, mosquitoes, diversity
Mosquitoes (Diptera: Culicidae) are probably the most medically important group of arthropods worldwide because of the ability of some species to transmit pathogens to humans (Clements 2011), causing major health issues in some parts of the world. Mosquito-borne diseases are frequent in French Guiana with malaria occurring mainly in inland areas, dengue and chikungunya in urban areas, while many lesser known crypto-arboviruses occur in sylvan and/or rural environments (
French Guiana is mainly covered by primary rainforest and its inhabitants (ca. 250,000) are mostly distributed along the coast (
This work is an ongoing process and should help to understand mosquito distribution across French Guiana. This database will also be used to disseminate biodiversity information related to future studies on mosquito distribution in French Guiana in general and in medical entomology and ecology in particular. We aim to promote the best practices for recording and sharing biodiversity data within our research community, and highly encourage foreign institutions to do the same. Our goal is to provide data on Guianese mosquitoes and to make available a fast and efficient tool for sharing and tracking reliable information on specimens in the form of an online database.
Description: This database concerns all mosquito (Diptera: Culicidae) species inhabiting French Guiana. Most specimens have been identified to species level or at least to genus level. The identifications were made by the first author based most of the time on the examination of immature and adult specimens, and by using the latest taxonomic publications on the genus or on the subgenus concerned (e.g.
Until now, the database was mostly filled with data from studies conducted on mosquitoes breeding in phytotelmata, which explains why the Sabethini are particularly well represented in the current dataset (Fig.
Taxonomic coverage by tribe (pie chart on the left) with a focus on the distribution of specimens by genus for the tribe Sabethini (pie chart on the right) from the dataset the “Mosquitoes of French Guiana” up to 2015. Because there are no tribes in the Anophelinae, they are represented at the subfamily level on the pie chart.
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Diptera
Family: Culicidae
Subfamilies: Anophelinae, Culicinae.
Tribes: Aedeomyiini, Aedini, Culicini, Mansoniini, Orthopodomyiini, Sabethini, Toxorhynchitini, Uranotaeniini.
Genera: Aedeomyia, Aedes, Anopheles, Chagasia, Coquillettidia, Culex, Deinocerites, Haemagogus, Johnbelkinia, Limatus, Lutzia, Mansonia, Onirion, Orthopodomyia, Psorophora, Runchomyia, Sabethes, Shannoniana, Toxorhynchites, Trichoprosopon, Uranotaenia, Wyeomyia.
Description: French Guiana (83,534 km²) is a French overseas region situated in South America at the eastern limit of the Guiana Shield. The latter is a mountainous tableland extending, from West to East, across Guyana, Suriname, French Guiana, as well as parts of Colombia, Venezuela and Brazil. The sampling area is delimited by the current administrative boundaries of the territory of French Guiana (Fig.
Geographical methods: GPS coordinates were obtained using a Garmin GPSmap 60CSx device or higher equivalent of the GPSmap series. The World Geodetic System 1984 (WGS 84) was used as geodetic system and associated with UTM 21-22 N for map projection.
Coordinates: 2°5'24"N and 5°50'60"N Latitude; 54°36'36"W and 51°31'48"W Longitude
Notes: From October 2013 to present.
Title: Mosquitoes of French Guiana
Personnel: Stanislas Talaga
Study area descriptions: Collecting trips were conducted in various locations throughout French Guiana ranging from urban to pristine environments.
Design description: This database was originally built from studies on mosquito-phytotelm associations at the scale of French Guiana. Immature mosquitoes were collected from at least 30 water-holding structures per phytotelm species, per locality. However, the extent of the sampling area was not standardized between the different localities. The database also contains some records of opportunistically sampled immature and adult mosquitoes conducted by the first author.
Funding: Data for this resource have been obtained within the framework of the projects BIOHOPSYS and DIADEMA from the CEBA (CEnter for the study of Biodiversity in Amazonia) and thanks to a PhD fellowship from the Université Antilles-Guyane awarded to Stanislas Talaga. CEBA is funded by an Investissement d’Avenirgrant managed by the French
Study extent description: Study sites were located throughout French Guiana.
Sampling description: The following techniques were used; however, not all techniques were used at every collecting site and the sampling design may not have been the same at all sites.
Immature container mosquitoes were collected by extracting plant-held water using a great variety of sucking devices in order to fit the great variety of plant structures and water volumes. On some occasions, natural and artificial ovitraps were used, including bamboo stumps, CDC ovitraps and artificial bromeliads installed at ground or canopy level. Immature mosquitoes from larger bodies of water were collected by using a kick net. Adult mosquitoes were attracted in the field by human bait and captured with a butterfly net or with an entomological aspirator when they alighted.
Processing: Whenever possible, samples were brought back alive to the laboratory. Immature mosquitoes were individually reared in 2 mL Eppendorf tubes and placed in a climatic chamber at 28 °C to obtain adults. When a sufficient number of adults was obtained, some of them were stored in individual tubes containing 95% ethanol. Fourth instar and pupal skins were also sorted and stored in individual tubes containing 70% ethanol. Laboratory-reared adults and adults issued from field capture were killed by freezing. Three legs from the right side of each specimen were then carefully dissected and kept in a separate vial containing 95% ethanol and stored at -20 °C for further molecular investigations. Adults were mounted on their right side on a pin point attached to a No. 3 stainless steel insect pin and stored in entomological boxes. Specimen codes are based on the name of the collection followed by a unique serial number as proposed by
Selected specimens were photographed using a Leica DFC450 camera mounted on a Leica MZ16 macroscope under a light dome simulating natural light. Images were Z-stacked using the Leica LAS Z-stacking module. Montage pictures and collecting information for each specimen are stored in an online Voseq database (
Sample data entry of our online database (http://mosquitoes.ups-tlse.fr with restricted access) holding the “Mosquitoes of French Guiana” dataset.
Specimens are initially curated at the Ecofog laboratory by Stanislas Talaga and can be deposited in museums for further taxonomic study.
Quality control description: Considering different sources of GPS errors (such as ionosphere delay and signal multipath), we estimate the accuracy of the coordinates to be around 30 meters at a 95% confidence level.
Dataset title: Mosquitoes of French Guiana
Resource: r=mosquitoes_of_french_guiana
Character encoding: UTF-8
Format name: Darwin Core Archive (
Format version: 1.0
Distribution: http://130.120.204.55:8080/ipt/resource.do?r=mosquitoes_of_french_guiana
Publication date of data: 2015-06-12
Language of database: English
License of use: Other
Date of metadata creation: 2014-12-10
Hierarchy level: Dataset
IP rights notes: This work is licensed under a Creative Commons Attribution- NonCommercial 4.0 International Public License. http://creativecommons.org/licenses/by-nc/4.0/ Users of this resource should also comply with the CEBA data sharing agreement available here: http://www.labex-ceba.fr/assets/CEBA_Data_Sharing_Agreement_nov2013.pdf
We would like to thank Pierre Solbes and Sébastien Cally from the EDB laboratory for technical support, Marceau Minot, Frédéric Petitclerc and the team of the