Research Article |
Corresponding author: Qiulei Men ( menqiulei888@126.com ) Academic editor: Christopher Borkent
© 2015 Qiulei Men, Guoxi Xue, Hai Yang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Men Q-L, Xue G-X, Yang H (2015) Two new species of the genus Nephrotoma (Diptera, Tipuloidea, Tipulidae) from China with a key to species from Mainland China. ZooKeys 532: 117-136. https://doi.org/10.3897/zookeys.532.5970
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Nephrotoma liankangensis sp. n. and N. pseudoliankangensis sp. n. are described from males and females collected from Henan and Yunnan provinces, China. Morphological descriptions and illustrations for the new species are provided. A key to known species from mainland China is provided. Some internal reproductive structures, including male semen pump, female vaginal apodeme and spermatheca, are described and compared. The possible usefulness of these internal reproductive structures for separating related species is analyzed. The type specimens are deposited in the animal specimen room, School of Life Sciences, Anqing Normal University, Anhui Province, China.
Nematocera , crane flies, taxonomy, internal reproductive organ, China
Nephrotoma is a large genus in the family Tipulidae. It was originally erected by
During this study of crane fly specimens collected from Henan and Yunnan provinces, China, two new species of Nephrotoma were found. Morphological descriptions and detailed illustrations for the new species are provided herein. A key to known species from mainland China is provided. In addition, some internal reproductive structures of the new taxa, including male semen pump, female vaginal apodeme and spermatheca, are described and compared. The possible usefulness of these internal reproductive structures for separating related species is analyzed.
The specimens examined in this study were collected from Henan and Yunnan provinces by the first author and undergraduates of the School of Life Sciences, Anqing Normal University. The genitalia, including male hypopygium and female ovipositor were removed and soaked in 10% NaOH for 12 hours to clear the muscle for examination. The cleared genitalia were immersed in glycerin jelly, and then examined and drawn using Leica MZ125 (Leica, Germany) stereomicroscope. All measurements were made with the aid of a digital caliper in millimeters (mm). The terminology and methods of description and illustration follow that of
The key was principally constructed from descriptions in the literature without examination of the type species of most of these species, and should be considered preliminary. The characters used in the key rely primarily on the structure of genitalia, the variation of veins and the number of stripes on the prescutum.
1 | Prescutum with stripe(s) | 2 |
– | Prescutum without stripe | 3 |
2 (1) | Prescutum with only one broad stripe, which almost covers the whole prescutum (see |
N. villosa (Savchenko, 1973) (China: Anhui) |
– | Prescutum with more than one stripe | 5 |
3 (1) | Stigma covered with macrotrichia | 4 |
– | Stigma not covered with macrotrichia (see |
N. hypogyna Yang & Yang, 1990 (China: Yunnan) |
4 (3) | Head and thorax brown with grayish-white pruinosity; wing entirely light brown (see |
N. guangxiensis Yang & Yang, 1993 (China: Guangxi) |
– | Head yellow, thorax brown, without grayish-white pruinosity; wing hyaline, tinged with light brown at apex (see |
N. jinxiuensis Yang & Yang, 1993 (China: Guangxi) |
5 (2) | Prescutum with black stripes | 6 |
– | Prescutum with stripes not black | 7 |
6 (5) | Prescutal stripes with velvety black margin | 29 |
– | Prescutal stripes without velvety black margin | 28 |
7 (5) | Prescutum with four stripes | 8 |
– | Prescutum with three stripes | 9 |
8 (7) | Male tergite nine without median notch, medially terminated into an obtuse lobe (see |
N. rectispina Alexander, 1925 (China: Hubei, Guizhou) |
– | Male tergite nine with U-shaped notch; flagellum black throughout | 10 |
9 (7) | Abdomen without dark apical or lateral stripes | 11 |
– | Abdomen with dark apical and/or lateral stripes | 12 |
10 (8) | Male tergite nine produced into two lobes; pleura yellow variegated with pale yellow (see |
N. quadrinacrea Alexander, 1949 (China: Hubei, Guangdong) |
– | Male tergite nine produced into four lobes; pleura entirely light yellow (see |
N. progne Alexander, 1949 (China: Guangdong) |
11 (9) | Male tergite nine projected into four lobes | 13 |
– | Male tergite nine projected into two lobes | 14 |
12 (9) | Occiput with mark | 17 |
– | Occiput without mark | 18 |
13 (11) | Caudal margin of male sternite eight with an appendage directed caudally (see |
N. meridionalis Yang & Yang, 1997 (China: Hainan, Guangxi) |
– | Caudal margin of male sternite eight without appendage; abdomen uniformly coloured | 15 |
14 (11) | Cell m1 petiolate (see |
N. inorata Alexander, 1951 (China: Xizang; India) |
– | Cell m1 sessile; male sternite eight with produced appendage at caudal margin | 16 |
15 (13) | Occiput without mark (see |
N. sichuanensis Yang & Yang, 1990 (China: Sichuan) |
– | Occiput with a linear mark medially; male sternite nine with an appendage bifid and directed caudally (see |
N. koreana Tangelder, 1984 (China: Hebei, Heilongjiang, Ningxia; Russia; North Korea) |
16 (14) | Flagellum dark yellow; scutum with spots brown; abdomen dark yellow (see |
N. qinghaiensis qinghaiensis Yang & Yang, 1987 (China: Qinghai) |
– | Flagellum with first flagellomere yellowish brown, the remainder of flagellum brown; scutum with black spots; abdomen blackish brown (see |
N. qinhaiensis nigrabdomen Yang & Yang, 1990 (China: Heilongjiang, Inner Mongolia) |
17 (12) | Cell m1 sessile | 24 |
– | Cell m1 petiolate | 25 |
18 (12) | Flagellomeres bicoloured, dark brown on enlarged bases, brown on apical portions of each flagellomere (see |
N. flavonota (Alexander, 1914) (China: Zhejiang, Fujian, Hainan; Japan) |
– | Flagellomeres uniformly coloured | 19 |
19 (18) | Flagellum with first flagellomere yellow, the remainder of flagellum black or brown | 20 |
– | Flagellum entirely black | 21 |
20 (19) | Inner gonostylus with toothed crest (see |
22 |
– | Inner gonostylus without crest (see |
23 |
21 (19) | Wing weak brown; prescutum with orange stripes (see |
N. citricolor Alexander, 1949 (China: Fujian) |
– | Wing whitish hyaline; prescutum with fuscous stripes (see |
N. makiella (Matsumura, 1916) (China: Fujian, Taiwan) |
22 (20) | Male sternite eight without produced appendage at caudal margin (see |
N. profunda Alexander, 1935 (China: Sichuan, Hubei) |
– | Male sternite eight with produced appendage at caudal margin (see |
N. hunanensis Yang & Yang, 1991 (China: Hunan) |
23 (20) | Male tergite nine with two horn-shaped processes (see |
N. ruiliensis Yang & Yang, 1990 (China: Yunnan) |
– | Male tergite nine without horn-shaped processes (Fig. |
N. pseudoliankangensis sp. n. (China: Yunnan) |
24 (17) | Vertex with a triangular stripe; inner gonostylus with a spinous lobe at posterior basal portion (see |
N. stylacantha Alexander, 1937 (China: Fujian, Jiangsu) |
– | Vertex with a thin linear stripe; inner gonostylus without lobe described as above (see |
N. alticrista Alexander, 1941 (China: Sichuan) |
25 (17) | Male tergite nine without notch at caudal margin, medially terminated into an obtuse lobe (see |
N. medioproducta Alexander, 1940 (China: Zhejiang) |
– | Male tergite nine with notch in the middle of caudal margin | 26 |
26 (25) | Male tergite nine with two spinous processes excluding setae (see |
N. sinensis (Edwards, 1916) (China: Beijing, Henan, Jiangsu, Shaanxi, Sichuan, Jiangsu, Yunnan, Hainan, Taiwan; Russia; North Korea; South Korea) |
– | Male tergite nine without spinous process excluding setae (see |
27 |
27 (26) | Pleura yellow with a variable amount of red; male tergite nine with a V-shaped notch in the middle of caudal margin (see |
N. omeiana Alexander, 1935 (China: Sichuan, Hubei, Taiwan) |
– | Pleura yellowish brown with a variable amount of white; male tergite nine with a rounded notch in the middle of caudal margin (Fig. |
N. liankangensis sp. n. (China: Henan) |
28 (6) | Scutum uniformly coloured | 30 |
– | Scutum with dark stripe or spot, or with different colours in middle portion | 31 |
29 (6) | Cell m1 petiolate (see |
66 |
– | Cell m1 sessile (see |
N. catenata catenata Alexander, 1935 (China: Sichuan) |
30 (28) | Abdomen not uniformly coloured, with dark stripe on tergites | 32 |
– | Abdomen uniformly coloured (see |
N. basiflava Yang & Yang, 1991 (China: Qinghai) |
31 (28) | Occiput without a mark | 34 |
– | Occiput with a mark | 35 |
32 (30) | Cell m1 sessile (see |
N. drakanae Alexander, 1936 (China: Gansu, Inner Mongolia) |
– | Cell m1 petiolate | 33 |
33 (32) | Occiput without a mark; scutellum orange ochreous (see |
N. parva (Edwards, 1916) (China: Jiangxi, Guangxi, Guangdong, Taiwan) |
– | Occiput with triangular black spot; scutellum black (see |
N. distans Edwards, 1928 (China: Xizang) |
34 (31) | Antennae black throughout; abdomen chiefly black, with fourth, fifth and base of third tergites orange (see |
N. aurantiocincta Alexander, 1941 (China: Sichuan, Yunnan) |
– | Antennae variously coloured but not black throughout; abdomen chiefly yellow, with fourth and fifth tergites not orange | 36 |
35 (31) | Flagellum with basal three segments yellow (see |
N. catenata guizhouensis Yang & Yang, 1988 (China: Guizhou) |
– | Flagellum with basal segments not yellow, or only first segment yellow | 42 |
36 (34) | Abdomen with seventh segment black | 37 |
– | Abdomen with seventh segment not black | 38 |
37 (36) | Cell m1 long-petiolate, longer than m-m (see |
N. hainanica Alexander, 1936 (China: Hainan) |
– | Cell m1 short-petiolate, shorter than m-m (see |
N. biarmigera Alexander, 1935 (China: Zhejiang) |
38 (36) | Male sternite eight without produced appendage in the middle of caudal margin (see |
39 |
– | Male sternite eight with produced appendage in the middle of caudal margin (see |
40 |
39 (38) | Process of male tergite nine widened basally and narrowed apically (see |
N. tianlinensis Yang & Yang, 1993 (China: Guangxi) |
– | Process of male tergite nine horn-shaped (see |
41 |
40 (38) | Male sternite nine with an appendage directed ventrally in the middle of caudal margin (see |
N. zhejiangensis Yang & Yang, 1995 (China: Zhejiang) |
– | Male sternite nine without appendage (see |
N. geniculata Yang & Yang, 1987 (China: Inner Mongolia, Hubei, Sichuan, Guizhou) |
41 (39) | Abdominal tergites with median stripe; inner gonostylus without crest (see |
N. cuneata Yang & Yang, 1991 (China: Hubei, Hunan) |
– | Abdominal tergites without median stripe; inner gonostylus with crest ( |
N. xichangensis Yang & Yang, 1990 (China: Sichuan) |
42 (35) | The first flagellomere not the same colour as remaining flagellomeres of flagellum | 43 |
– | Flagellum with flagellomeres all the same colour | 44 |
43 (42) | Inner gonostylus with crest | 45 |
– | nner gonostylus without crest | 46 |
44 (42) | Male tergite nine without emargination, or with shallow emargination on caudal margin | 53 |
– | Male tergite nine with U-shaped or V-shaped notch on caudal margin | 54 |
45 (43) | Crest of inner gonostylus with tooth on dorsal margin | 47 |
– | Crest of inner gonostylus without tooth (see |
N. hirsuticauda Alexander, 1924 (China: Heilongjiang, Inner Mongolia, Gansu, Ningxia; Russia; Japan; Mongolia; North Korea) |
46 (43) | Caudal margin of male tergite nine produced into a pair of flattened black lobes, their medial edges coarsely toothed; outer gonostylus with tip curved and subacute, the margin of gonostylus with three or four teeth (see |
N. nigrostylata Alexander, 1935 (China: Hubei, Zhejiang, Fujian, Sichuan, Guizhou, Guangxi) |
– | Caudal margin of male tergite nine, and outer gonostylus not as above | 48 |
47 (45) | Outer gonostylus abruptly narrowed at apical half; inner gonostylus with a relatively short beak (see |
N. relicta (Savchenko, 1973) (China: Heilongjiang, Sichuan, Hubei; Russia; North Korea; South Korea; Mongolia; Finland) |
– | Outer gonostylus gradually narrowed; inner gonostylus with a relatively long beak (see |
N. parvirostra Alexander, 1924 (China: Beijing, Hebei, Heilongjiang, Hubei, Sichuan; Russia; Mongolia; South Korea; Japan) |
48 (46) | Abdominal tergites without median stripe (see |
N. evittata Alexander, 1935 (China: Sichuan, Yunnan) |
– | Abdominal tergites with median stripe | 49 |
49 (48) | Male sternite eight with appendage in the middle of caudal margin (see |
50 |
– | Male sternite eight without appendage in the middle of caudal margin (see |
51 |
50 (49) | Male tergite nine with lobes acute apically (see |
N. concava Yang & Yang, 1990 (China: Gansu) |
– | Male tergite nine with lobes rounded apically (see |
N. hubeiensis Yang & Yang, 1987 (China: Hubei) |
51 (49) | Male tergite nine with two blunt processes on each side of hind margin (see |
N. xizangensis Yang & Yang, 1987 (China: Xizang) |
– | Male tergite nine with two acute processes on each side of hind margin | 52 |
52 (51) | Occipital mark brown, rounded; abdomen with segments seven to nine dark brown (see |
N. repanda (Alexander, 1914) (China: Sichuan; Russia; North Korea; South Korea; Japan) |
– | Occipital mark black, subtriangular; abdomen with all segments yellow (see |
N. retenta Alexander, 1935 (China: Sichuan-Xizang border) |
53 (44) | Male tergite nine with process not directed caudally (see |
N. libra Alexander, 1951 (China: Xizang) |
– | Male tergite nine with process directed caudally (see |
55 |
54 (44) | Tergite nine dividing into four processes | 56 |
– | Tergite nine dividing into two processes | 57 |
55 (53) | Vertex with a light brown spot between eyes; male sternite nine with an appendage directed caudally in the middle of hind margin (see |
N. didyma Yang & Yang, 1987 (China: Xizang) |
– | Vertex with a black spot between eyes; male sternite nine without appendage in the middle of hind margin (see |
N. claviformis Yang & Yang, 1987 (China: Xizang) |
56 (54) | Inner gonostylus with crest | 58 |
– | Inner gonostylus without crest | 59 |
57 (54) | Male sternite eight with appendage in the middle of caudal margin | 67 |
– | Male sternite eight without appendage in the middle of caudal margin | 68 |
58 (56) | Inner gonostylus produced caudad into a long tail-like portion (see |
N. nigrohalterata Edwards, 1928 (China: Sichuan, Xizang) |
– | Inner gonostylus without such tail-like portion; sternite eight with an appendage in the middle of caudal margin | 60 |
59 (56) | Male sternite eight with appendage in the middle of caudal margin (see |
N. aculeata (Loew, 1871) (China: Heilongjiang, Shanxi; widely distributed in Palaearctic Region) |
– | Male sternite eight without appendage in the middle of caudal margin | 62 |
60 (58) | Occipital mark dark brown; male sternite nine with appendage in the middle of caudal margin (see |
N. virgata (Coquillett, 1898) (China: Anhui, Hebei, Hubei, Sichuan, Zhejiang; Russia; North Korea; South Korea; Japan) |
– | Occipital mark black; male sternite nine without appendage in the middle of caudal margin | 61 |
61 (60) | Flagellum entirely brown; male sternite eight with a curved appendage directed ventrally at caudal margin (see |
N. ocellata Yang & Yang, 1990 (China: Sichuan) |
– | Flagellum entirely black; male sternite eight with a straight appendage directed caudad at caudal margin (see |
N. cornicina cornicina (Linnaeus, 1758) (China: as far south as Zhejiang; widely distributed in Palaearctic Region) |
62 (59) | Caudal margin of male tergite nine with two intermediate rounded processes and two spinous processes laterally (see |
N. kunagi Yang & Yang, 1990 (China: Sichuan) |
– | Caudal margin of male tergite nine with processes not as above | 63 |
63 (62) | Cell m1 petiolate (see |
N. pleuromaculata Alexander, 1927 (China: Hubei, Sichuan, Yunnan; India) |
– | Cell m1 sessile | 64 |
64 (63) | Intermediate lobes of male tergite nine with apex further produced into a pale triangular point (see |
N. martynovi Alexander, 1935 (China: Hebei, Heilongjiang, Inner Mongolia; Russia; Mongolia) |
– | Intermediate lobes of male tergite nine with apex lacking such a point | 65 |
65 (64) | Male tergite nine with a slender glabrous spine arising from the ventrolateral portion (see |
N. scalaris parvinotata (Brunetti, 1918) (China: Beijing, Hebei, Shanxi, Shandong, Xinjiang, Inner mongolia, Gansu, Ningxia, Heilongjiang, Jiangsu, Anhui, Sichuan, Guizhou; Russia; Georgia; Armenia; Azerbaijan; Turkey; Syria; Lebanon; Iran; Kazakhstan; Turkmenistan; Uzbekistan; Tajikistan; Kyrgyzstan; Afghanistan; Mongolia; India; Pakistan) |
– | Male tergite nine without such a spine arising from the ventrolateral portion (see |
N. pullata (Alexander, 1914) (China: Heilongjiang; Russia; North Korea; Japan) |
66 (29) | Male sternite eight with a small compressed lobe at posterior margin (see |
N. pilata Alexander, 1935 (China: Sichuan) |
– | Male sternite eight lacking such a lobe at posterior margin (see |
N. vesta Alexander, 1949 (China: Guangdong) |
67 (57) | Abdominal tergites black with pale yellow lateral stripes | 69 |
– | Abdominal tergites yellow with dark lateral stripes | 70 |
68 (57) | Inner gonostylus without crest | 71 |
– | Inner gonostylus with crest | 72 |
69 (67) | Antennae entirely black; male tergite nine terminating in two rounded lobes at caudal margin (see |
N. biformis Alexander, 1935 (China: Sichuan) |
– | Antennae entirely brown; male tergite nine terminating in two acute lobes at caudal margin (see |
N. joneensis Yang & Yang, 1990 (China: Gansu) |
70 (67) | Cell m1 petiolate (see |
N. barbigera (Savchenko, 1964) (China: Heilongjiang, Jilin, Gansu, Ningxia, Shanxi; Russia) |
– | Cell m1 sessile | 77 |
71 (68) | Male tergite nine with a pair of spinous processes except setae | 73 |
– | Male tergite nine without spinous processes except setae (see |
N. bifusca Alexander, 1920 (China: Heilongjiang, Hebei, Jilin; Russia; North Korea; South Korea; Japan) |
72 (68) | Cell m1 petiolate | 75 |
– | Cell m1 sessile | 76 |
73 (71) | Male tergite nine projected into a pair of spike-like processes at caudal margin, each with a series of six or seven blackened points along their medial edge (see |
74 |
– | Male tergite nine projected into a pair of fingerlike processes at caudal margin, the horn without points described as above (see |
N. shanxiensis Yang & Yang, 1990 (China: Shanxi) |
74 (73) | Cell m1 petiolate (see |
N. impigra impigra Alexander, 1935 (China: Zhejiang, Hubei, Jiangxi, Fujian, Sichuan) |
– | Cell m1 sessile (see |
N. impigra fulvovittata (Savchenko, 1964) (China: Yunnan) |
75 (72) | Male tergite nine terminating in two angular lobes at caudal margin (see |
N. quadristriata (Schummel, 1833) (China: Xinjiang; widely distributed in Palaearctic Region) |
– | Male tergite nine terminating in two truncated lobes at caudal margin (see |
N. spicula Tangelder, 1984 (China: Heilongjiang; Russia; North Korea) |
76 (72) | Inner gonostylus with crest toothed (see |
N. scurra (Meigen, 1818) (China: Gansu, Inner Mongolia; widely distributed in Palaearctic Region) |
– | Inner gonostylus with crest not toothed (see |
78 |
77 (70) | Male tergite nine terminating in two truncated lobes at caudal margin; inner gonostylus with an angular process on dorsal side (see |
N. ligulata Alexander, 1925 (China: Xinjiang; Russia; Turkmenistan; Uzbekistan; Tajikistan; Kyrgyzstan; Afghanistan; Mongolia; India) |
– | Male tergite nine terminating in two bluntly rounded lobes at caudal margin; inner gonostylus without process on dorsal side (see |
N. xinjiangensis Yang & Yang, 1987 (China: Xinjiang) |
78 (76) | Male sternite nine with an appendage directed caudally (see |
N. kaulbacki Alexander, 1951 (China: Xizang) |
– | Male sternite nine with an appendage directed ventrally (see |
79 |
79 (78) | Abdominal tergites without lateral stripes; male tergite nine separated by a U-shaped notch medially on caudal margin (see |
N. perobliqua Alexander, 1936 (China: Gansu) |
– | Abdominal tergites with lateral stripes; male tergite nine separated by a V-shaped notch medially on caudal margin (see |
N. pjotri Tangelder, 1984 (China: Xinjiang; Kazakhstan; Uzbekistan; Kyrgyzstan) |
Antennae with flagellum light brown, pleura light yellow conspicuously patterned with white, abdominal tergites with two black lateral stripes and one brown median stripe, ninth tergite with two rounded lobes which are densely covered with black spines, posterior margin of ninth tergite not concaved at base of lobes.
Male (n=2): body length 9.8 mm, wing 10.5 mm, antenna 5.8 mm.
Nasus yellow with black setae, palpi black. Antennae relatively long, if bent backward extending to the first abdominal tergite, scape and pedicel yellow, flagellum 10-segmented, with the first flagellomere yellow, with the second to tenth flagellomeres light brown and enlarged at both ends, the basal enlargement black with black setae basally, subequal to the flagellomeres from which they arise. Head yellow, occipital brand brown and narrow, along the middle line of occiput (Fig.
Nephrotoma liankangensis sp. n. 1 head, dorsal view 2 thorax, dorsal view 3 left wing 4 hypopygium, lateral view 5 hypopygium, ventral view 6 ninth tergite, dorsal view 7 outer gonostylus, lateral view 8 inner gonostylus, lateral internal view 9 inner gonostylus, lateral external view. Abbreviations: i gonst = inner gonostylus, o gonst = outer gonostylus, S = stergite.
Pronotum light brown, changing into yellow in middle. Prescutum yellow with three brown stripes, median one expanded apically, not extending to the hind border, lateral stripe straight and rounded apically, extending slight beyond the middle of median stripe (Fig.
Abdomen generally yellow, the first tergite light brown, tergites two to six with a light brown median stripe and two black lateral stripes, sternites two to seven with light brown median stripe, hypopygium chiefly yellowish brown. Male hypopygium (Figs
Aedeagal guide horn-shaped in lateral view, very acute apically (Fig.
Nephrotoma liankangensis sp. n. 10 aedeagal guide, lateral view 11 aedeagal guide, dorsal view 12 semen pump, dorsal view 13 semen pump, lateral view 14 compressor apodeme 15 ovipositor, lateral view 16 ovipositor, dorsal view 17 ninth stergite and vaginal apodeme, dorsal view 18 spermatheca, dorsal view 19 spermatheca, lateral view. Abbreviations: aed gd = aedeagal guide, pm = paramere, ventr app = ventral appendage of aedeagal guide, PIA = posterior immovable apodeme, AIA = anterior immovable apodeme, CA = compressor apodeme, me rdg = median ridge, T = tergite, cerc = cercus, hyva = hypovalva.
Semen pump with posterior immovable apodeme (PIA) narrow, dorsally bent in lateral view (Fig.
Female (n=2): body length 16.2 mm, wing 12.0 mm, antenna 3.2 mm.
The general coloration of head, thorax and abdomen similar to that of male.
Antennae relatively short, if bent backward not extending to abdomen, scape and pedicel yellow, flagellum 10-segmented, each flagellomere cylindrical, gradually shorter and slightly enlarged at base, the basal two flagellomeres yellow, three to ten flagellomeres yellowish brown with black at base.
Ovipositor yellowish brown, ninth sternite very thin, lanceolate, separated medially (Fig.
Vaginal apodeme widened at basal half and gradually tapered to the end, very acute apically (Fig.
Holotype male. Pinned specimen. China: Henan Province, Xin County, Liankangshan National Nature Reserve, 18 Jul. 2014, coll. Qiulei Men. Paratype. Pinned specimen. China: 1 male 2 females, same data as holotype, coll. Wu Zeng.
China (Henan).
The specific epithet is a noun ‘liankang’ with Latin suffix ‘ensis’, referring to the distribution of the new species.
This new species is similar to N. pseudoliankangensis, as discussed below, and another Chinese species, N. sinensis (Edwards, 1916), by the coloration of the prescutum and wings, the shape of lobes on the ninth tergite and the shape of inner gonostylus. It can be easily distinguished from the latter by the prescutum not bearing a black spot on anterior portion of the lateral stripe (this black spot present in N. sinensis as described by
General coloration light yellow, antennae with flagellum black except the first flagellomere, pleura white conspicuously patterned with yellow, abdominal tergites with two black lateral stripes and one brown median stripe, ninth tergite with two rounded lobes, posterior margin of ninth tergite slightly concaved at base of lobes.
Male (n=2): body length 9.6 mm, wing 11.0 mm, antenna 5.1 mm.
Nasus brown with brown setae, palpi black. Antennae relatively long, if bent backward extending to the first abdominal tergite, scape and pedicel yellow, flagellum 10-segmented, with the first flagellomere yellow, with second to tenth flagellomeres black and enlarged at base and apex, each flagellomere with black setae at base, subequal to the flagellomeres where they are found. Head yellow without occipital brand (Fig.
Pronotum entirely yellow. Prescutum yellow with three brown stripes, median one percurrent and expanded apically, lateral stripe straight and rounded apically, the area between lateral stripe and lateral border of prescutum suffused with brown (Fig.
Abdomen generally yellow, the first segment yellow with light brown tergite, tergites two to seven with a brown median stripe and two black lateral stripes, the median stripe expanded at hind border, tergite eight entirely brownish black; sternites two to seven with light brown median stripe, hypopygium chiefly yellowish brown. Male hypopygium (Figs
Aedeagal guide very similar to that of N. liankangensis, with paramere not blunt apically, the dorsal margin slightly arched, the ventral and outer margins forming an obtuse angle (Fig.
Nephrotoma pseudoliankangensis sp. n. 28 aedeagal guide, lateral view 29 semen pump, dorsal view 30 semen pump, lateral view 31 compressor apodeme 32 ovipositor, lateral view 33 ovipositor, dorsal view 34 ninth stergite and vaginal apodeme, dorsal view 35 spermatheca, dorsal view 36 spermatheca, lateral view.
Semen pump very similar to that of N. liankangensis, with posterior immovable apodeme (PIA) not bent directed dorsad at the apex in lateral view (Fig.
Female (n=3): body length 15.8 mm, wing 13.0 mm, antenna 2.5 mm.
The colouration of head, thorax and abdomen similar to those of male.
Antennae relatively short, scape and pedicel yellow, flagellum 10-segmented, each flagellomere cylindrical, gradually shorter, the basal two flagellomeres entirely yellow, three to ten flagellomeres yellowish brown with black at base.
Ovipositor (Figs
Vaginal apodeme widened at basal two fifths, the rest of vaginal apodeme tubular, parallel, acute apically (Fig.
Spermatheca spherical, brown, well-sclerotized, with membranous extension truncate (Figs
Holotype male. Pinned specimen. China: Yunnan Province, Kunming, Baofeng wetland park, 31 Aug. 2013, coll. Bin Zhang. Paratype. Pinned specimen. China: 1 male 3 females, same data as holotype.
China (Yunnan).
The specific epithet is based on the name of the related species, N. liankangensis, with the Latin prefix ‘pseudo’, referring to the morphological similarity of the new species to N. liankangensis.
This new species is externally similar to N. liankangensis by the colouration of head, thorax, abdomen and wings, and the shape of inner and outer gonostyli. It can be easily distinguished from the latter by antennae with the second to tenth flagellomeres black (the second to tenth flagellomeres yellow with basal enlargement black in N. liankangensis), the occiput unpatterned (with brown median brand in N. liankangensis as shown in Fig.
Based on morphological comparison, the characters of the vaginal apodeme, spermatheca and semen pump are uniform in different individuals of N. pseudoliankangensis and N. liankangensis. However, these structures show noticeable differences between the two species. More comparative morphological study may not only prove a high application value of the characters of these internal reproductive structures in separating relative species, but also prove the roles of these structures for phylogenetic studies as mentioned by
We wish to express sincere thanks to Dr. Pjotr Oosterbroek, University of Amsterdam, Amsterdam, the Netherlands, for his help with literature and for his valuable web site, the Catalogue of the Craneflies of the World (http://nlbif.eti.uva.nl/ccw/index.php), which provides much valuable information. Dr. Oosterbroek and Dr. Jon Gelhaus both provided helpful comments to a previous version of this manuscript. This study is supported by grants from the National Science Foundation of China (No. 31300551), the start-up grant of scientific research from Anqing Normal University (No. 044-K05000130005), and the Scientific Exploration of Liankangshan National Nature Reserve, Henan, China.