Research Article |
Corresponding author: Scott Monks ( 1scottmonks@hotmail.com ) Academic editor: David Gibson
© 2015 Christian E. Bautista-Hernández, Scott Monks, Griselda Pulido-Flores, Rafael Miranda.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Bautista-Hernández CE, Monks S, Pulido-Flores G, Miranda R (2015) A new species of Paracreptotrema (Digenea, Plagiorchiformes, Allocreadiidae) infecting two species of poeciliids in Río Malila of the Río Pánuco basin, Hidalgo, México, with a key to the species of the genus. ZooKeys 482: 55-66. https://doi.org/10.3897/zookeys.482.8144
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Paracreptotrema rosenthali sp. n. was discovered in the intestine of Xiphophorus malinche and Pseudoxiphophorus jonesii, collected from the headwaters of Río Malila, tributary of Río Conzintla, in the Río Pánuco basin, Hidalgo, México, during 2008–2009. The new species differs from the five known species of Paracreptotrema Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006 by having vitelline follicles that extend from a level anterior to the pharynx to mid-testes, the seminal vesicle which is more extensively folded, and a wider cirrus sac. The new species resembles P. heterandriae in the length of its ceca, which surpasses the posterior margin of the ovary but do not reach the testes. A key to the species of Paracreptotrema is provided.
Paracreptotrema , Xiphophorus malinche , helminth parasite, endemic species, key
Despite an increase in our knowledge of the helminth parasites of the species of fish in México,
Adult specimens of Xiphophorus malinche (60 individuals; May 2008 to July 2009) and Pseudoxiphophorus jonesii (Günther, 1874) (sensu
Comparison of morphological characteristics of the five species described as Paracreptotrema. Data for P. blancoi (México), P. blancoi (Costa Rica), P. mendezi and P. heterandriae taken from
P. blancoi | P. blancoi | P. mendezi |
P. profundulusi (text) |
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Body length | 465–732 (519) | 500–850 (688) | 680 | 600–990 (788) |
Maximum width | 200–387 (263) | 250–450 (349) | 310 | 287–500 (364) |
Oral sucker length × width | 70–90 (82) × 82.5–110 (90.4) | 100–155 (126.7) × 100–155 (126.2) | 100 × 120 | 102–150 (125) × 112–177 (137) |
Ventral sucker length × width | 162.5–207 (175.9) × 125–210 (173.8) | 120–175 (153.2) × 130–205 (174) | 170 × 170 | 165–250 (201) × 145–225 (189) |
Sucker ratios | ||||
Length | 1.7–2.4 (1:2.1) | 1.2–1.21 (1:1.2) | 1:1.4’ | 1.5–2.0 (1:1.6) |
Width | 1.4–2.5 (1:1.9) | 1.2–1.7 (1: 1.4) | 1.1–1.5 (1:1.4) | |
Pharynx length × width | 25–50 (40) × 30–62.5 (47.2) | 40–60 (50) × 45–75 (57) | 50 × 60 | 37–62 (48) × 37–70 (49) |
Ovary length × width | 37.5–77.5 (51.5) × 25–75 (40.7) | 55–110 (86.2) × 35–62.5 (69.2) | 27 × 74 | 47–125 (72) × 50–125 (90) |
Left testis length × width | 45–75 (64.2) × 37.5–70 (52.5) | 90–170 (132) × 70–120 (94.7) | 180 × 90 | 87–175 (124) × 75–125 (93) |
Right testis length × width | 42.5–87.5 (63.7) × 35–62.5 (52.5) | 89–167 (134) × 74–115 (95.1) | 170 × 90 | 87–175 (122) × 60–112 (92) |
Cirrus sac length × width | 62.5–137.5 (83.2) × 30–50 (37.5) | – × 35–62.5 (52) | 100 × 60 | 92–175 (135) × 37–95 (67) |
Eggs length × width | 45–60 (52) × 25–37.5 (32.5) | 52.5–62.5 (55.4) × 32.5–42.5 (38.5) | 46 × 37 | 50–62 (57) × 25–37 (31) |
Locality | Río Papagayo basin, Guerrero, México | Área de conservación, Guanacaste, Costa Rica | Lake Gatun, Panama | Río Tehuantepec basin, Oaxaca, México |
Host | Profundulus punctatus (Profundulidae) | Priapichthys annectens (Poeciliidae) | Brachyrhaphis episcopi (Poeciliidae) | Profundulus punctatus (Profundulidae) |
Reference |
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Sogandares-Bernal 1955 |
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P. profundulusi (table) |
P. heterandriae | P. rosenthali | |
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Body length | 675–990 (820) | 625–1,050 (783) | 720–940 (830) |
Maximum width | 287–500 (380) | 175–375 (252) | 350–550 (417) |
Oral sucker length × width | 115–137 (124) × 117–150 (133) | 100–160 (121) × 87–150 (113) | 105–160 (125) × 130–175 (140) |
Ventral sucker length × width | 175–250 (205) × 145–225 (186) | 112–195 (155) × 117–217 (163) | 170–203 (203) × 180–225 (205) |
Sucker ratios | |||
Length | 1.5–2.0 (1:1.7) | 0.9–1.6 (1:1.3) | 1:1.6 |
Width | 1.1–1.5 (1:1.3) | 1.2–1.7 (1:1.4) | 1:1.5 |
Pharynx length × width | 37–62 (48) × 37–70 (51) | 45–67 (54) × 50–75 (59) | 45–55 (52) × 38–70 (56) |
Ovary length × width | 47–125 (82) × 75–125 (98) | 55–112 (76) × 42–112 (72) | 63–135 (104) × 98–145 (111) |
Left testis length × width | 87–155 (122.4) × 62–112 (92) | 87–150 (119) × 52–125 (75) | 105–188 (136) × 68–168 (96) |
Right testis length × width | 87–162 (123.4) × 60–112 (99) | 87–150 (116) × 57–112 (79) | 110–188 (137) × 50–155 (88) |
Cirrus sac length × width | 100–175 (141) × 37–87 (69) | 100–145 (114) × 20–35 (28) | 75–110 (91) × 68–103 (80) |
Eggs length × width | 52–60 (57) × 25–30 (27.8) | 70–75 (72.5) × 35–41 (40) | 47–70 (52) × 25–45 (32) |
Locality | Río Tehuantepec basin, Oaxaca, México | Río la Antigua upper basin, Xalapa, Veracruz, México | Río Conzintla, Malila, Hidalgo |
Host | Profundulus punctatus (Profundulidae) | Heterandria bimaculata (Poeciliidae) | Xiphophorus malinche (Poeciliidae) |
Reference |
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Present study |
Paracreptotrema blancoi Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006
Holotype (CNHE 9263), 3 paratypes (CNHE 9264 to 9266), and 3 paratypes (HWML 75051 to 75054).
Paracreptotrema blancoi Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006 (CNHE–5315, Costa Rica; CNHE–7682, México); P. heterandriae Salgado-Maldonado, Caspeta-Mandujano & Martínez-Ramírez, 2012 (CNHE–8242); P. mendezi (Sogandares-Bernal, 1955) Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006 (HWML–22193, 22194); and P. profundulusi Salgado-Maldonado, Caspeta-Mandujano & Martínez-Ramírez, 2011 (CNHE–7684).
Xiphophorus malinche Rauchenberger, Kallman & Morizot (Poeciliidae). Vouchers deposited in MZNA fish collection, University of Navarra, Spain (
Río Malila, tributary of Río Conzintla, northeastern Hidalgo, México (20°44'N; 98°43'W).
Intestine.
Pseudoxiphophorus jonesii (Günther, 1874) (sensu
In X. malinche, 7 of 88 infected (7.9%). In P. jonesii, 1 of 36 infected (2.77%).
The species is named in honor of Gil G. Rosenthal, Department of Biology, Texas A&M University, College Station, Texas, and co-founder of the CICHAZ field station, for his friendship, contributions to the knowledge of species of Xiphophorus, and in recognition of his efforts to promote science in the Huasteca region of México.
[Based on 8 specimens] Body 720–940 (830 ± 83 n = 7) long, robust, aspinose. Anterior end rounded; body 350–550 (417 ± 66 n = 7), widening gradually, reaching maximum width at level of middle to posterior margin of acetabulum, terminating in narrower posterior end. Few, small, pigment spots in forebody. Oral sucker wider than long, subterminal, 105–160 (125 ± 20 n = 7) long, 130–175 (140 ± 16 n = 7) wide, with 2 papillae on posterior margin and several papillae along outer edge; opening subterminal, antero-ventrally directed. Average length of acetabulum, 170–230 (203 ± 18 n = 8) slightly greater than width, 180–225 (205 ± 15 n = 7), strongly muscular, sunken, visible externally by its rounded opening; tegument of acetabulum with fine striations radiating outward from acetabular opening. Ratio of length of oral sucker to length of acetabulum 1:1.4–1:2.0 (1:1.6) and width of oral sucker to width of acetabulum 1:1.3–1:1.7 (1:1.5). Prepharynx absent. Pharynx muscular, well developed, 45–55 (52 ± 4, n = 6) long, 38–70 (56 ± 11 n = 6) wide. Esophagus short, winding, 45–75 (57 ± 16, n = 3) long, 5–10 (n = 2) wide. Cecal bifurcation short distance anterior to acetabular margin. Ceca, mostly obscured by vitelline follicles, extend posterior to acetabulum, 725 (right side) to 800 (left side) long (n = 1), following curve of body, just surpassing posterior margin of ovary but not reaching testes (Fig.
The genus Paracreptotrema includes four species: P. blancoi Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006, P. mendezi (Sogandares-Bernal, 1955), P. profundulusi Salgado-Maldonado, Caspeta-Mandujano & Martínez-Ramírez, 2011, and P. heterandriae Salgado-Maldonado, Caspeta-Mandujano & Vázquez, 2012. The specimens of P. rosenthali sp. n. from X. malinche share the features established in the original concept of the genus (
Geographic barriers play an important role in the isolation of fish populations and their helminth fauna (
The distribution of X. malinche is restricted to the more northern Hidalgo anticline, separated from southern populations of fish by the barrier range of the Mexican plateau (
The papillae on the oral sucker were difficult to discern on our specimens. Two papillae on the posterior margin of the sucker were visible on some specimens, but only some of the papillae along outer edge were visible on a few specimens; thus, no papillae were included in the figure. We could discern several papillae along the outer edge of the oral sucker in specimens of P. blancoi, P. profundulusi, and P. heterandriae, but the entire complement of papillae was not visible in any specimens we examined. Study of specimens using scanning electron microscopy will be necessary for a full assessment of the number of papillae present, but the number of specimens available at this time is not sufficient for such a study.
All known species of Paracreptotrema have an oral sucker that is wider than long (Table
The presence of Laurer’s canal has been reported for the four previously known species. We were not able to discern the canal in specimens of the new species. The limited material precluded mounting of specimens in a more favorable position for observations of this structure, and no specimens were available for histological study. The populations of fish from which the specimens were collected are limited in size and fragile, and this helminth has not been found in other populations of fish close to the locality (
1 | Ceca do not surpass the anterior margin of the testes | 2 |
– | Ceca surpass the anterior margin of the testes | 4 |
2 | Anterior margin of the vitelline follicles does not reach the anterior margin of the pharynx | P. heterandriae |
– | Anterior margin of the vitelline follicles surpasses the anterior margin of the pharynx | 3 |
3 | Uterus extends to the posterior margin of the hindbody | P. mendezi |
– | Uterus does not extend to the posterior margin of the hindbody | P. rosenthali |
4 | Vitellarium extends posterior to the testes | P. profundulusi |
– | Vitellarium does not extend into the region posterior to the testes | P. blancoi |
We acknowledge the following people: Zachary W. Culumber, for help with the collection and identification of the fish; Roosevelt Rodríguez-Amador, who helped with the dissections; Luis García-Prieto and Scott L. Gardner (CNHE and HWML, respectively), for the loan of specimens; and Jorge Falcón-Ordaz for comments on an early version of this manuscript. Part of this manuscript was prepared during a postdoctoral research visit to the Harold W. Manter Lab by two of the authors (SM and GP-F), which was partially supported by funds from the Patronato Universitario (Gerardo Soza Castelán, President), Universidad Autónoma del Estado de Hidalgo, and the Consorcio de Universidades Mexicanas. Principal support was provided by the project “El efecto de hibridización en la diversidad de helmintos parásitos de peces del género Xiphophorus” (Clave 0127310) to SM, as was a scholarship from CONACYT to CEB-H (217861) during her PhD research project.