Research Article |
Corresponding author: Shuxia Wang ( cnmoths@163.com ) Academic editor: Erik J. van Nieukerken
© 2015 Tengteng Liu, Shuxia Wang, Houhun Li.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Liu T, Wang S, Li H (2015) Description of Telamoptilia grewiae sp. n. and the consequences for the definition of the genera Telamoptilia and Spulerina (Lepidoptera, Gracillariidae, Gracillariinae). ZooKeys 479: 121-133. https://doi.org/10.3897/zookeys.479.8899
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The new species Telamoptilia grewiae, reared from leafmines on Grewia biloba (Malvaceae) is described with details on adult and immature stages. The larval head and the pupa are described for the first time in Telamoptilia Kumata & Kuroko, 1988, and are illustrated with scanning electron micrographs and line drawings. Photographs of adult habitus, wing venation, male and female genitalia, as well as host plant and mines are provided. The apomorphic adult and larval characters of the new species in Telamoptilia are discussed in relation to the recognition of the genera Telamoptilia and Spulerina Vári, 1961.
Lepidoptera , Telamoptilia , new species, immature stage, leaf miner, China
The genus Telamoptilia Kumata & Kuroko, 1988 is globally represented by five species that may be found in the Oriental and African regions. The type species T. cathedraea (Meyrick, 1908) is geographically shared by the Oriental Region and Madagascar (
The larvae of Telamoptilia species are leaf miners. Three plant families are known as hosts for Telamoptilia: Malvaceae, Amaranthaceae and Convolvulaceae (
Telamoptilia grewiae sp. n. is associated with Malvaceae and is described in the present paper from adult external characters, male and female genitalia, wing venation and immature stages. The larval head and pupal features are described for the first time in Telamoptilia.
Field investigations were carried out in Mt. Baxian National Nature Reserves (40°11'N, 117°32'E), 300−600 m, Tianjin, China, from May to September in 2013 and June 2014. Leaves containing mines with larvae were placed in sealed plastic bags, or rearing containers with moist cotton. Larvae removed from mines were immersed in nearly boiling water for 30 seconds, and then were kept in 75% ethanol for morphological examination. Last instar larval skins, pupae, and exuviae were kept in 75% ethanol. Pupae in rearing containers were placed outdoors to overwinter, and were transferred into the laboratory at 20 °C on February 6, 2014. Emergence successively occurred from March 9 to early-April 2014. Adults were collected chiefly by rearing from immature stages, and occasionally by light trap.
Adult photographs were taken with a Leica M250A stereo microscope. Genitalia and wings were dissected and mounted according to the methods introduced by
Terminology of immature stages follows
All the specimens studied, including the types of the new species and the vouchered larvae and pupae, are deposited in the Insect Collection, Nankai University, Tianjin, China.
Adults (Figs
Variations. The white costal spot at about basal 1/10 is sometimes reduced to a small dot near the costa, the white costal stria at distal 3/10 is occasionally extended to unite the dot on the distal end of M3, the costal stria at distal 1/6 is sometimes wedge-shaped or absent, the white dot at the distal end of M3 sometimes moves to near distal end of cell, and the small white dots along termen sometimes are absent.
Venation (Fig.
Male genitalia (Figs
Wing venation and genitalia of Telamoptilia grewiae sp. n. 7 Wing venation, paratype, LTT12561W 8 Male genitalia, 8a, male genitalia with aedeagus detached, LTT12561 8b aedeagus, lateral view, same slide as 8a, 8c close-up of the distal portion of aedeagus, indicating the triangular process, dorsal view, LTT12257, 8d, eighth abdominal segment, paratype, same slide as 8a, 9 Female genitalia, paratype, LTT12555 (Scales = 0.2 mm except 8c = 0.1 mm).
Female genitalia (Fig.
Last instar larva (Figs
Head. Adfrontal area slightly convex above middle, AF setae placed on convexity (Fig.
Thorax. TI with XD, D, and SD setae placed on prothoracic shield, XD1 near anterior margin of prothoracic shield (Fig.
Abdomen. A1−8 with D1 and D2 closely approximated to each other, MD1 seta anterior and slightly dorsal to D1, SD1 posterior and dorsal to spiracle, SD2 shorter than SD1, anterior and dorsal to spiracle, closer to spiracle than SD1, L-group bisetose, L2 seta absent; A1 and A7−9 with SV-group unisetose, A2 and A6 bisetose, A3−5 trisetose; A9 with D1 short, MD1 seta anterior to D1, SD1 anterior and ventral to D2, as long as D2, SD1, L1, SV1 and V1 almost forming a line; A10 as shown in Fig.
Pupa (Figs
Pupal characters of Telamoptilia grewiae sp. n. 24 Pupa, lateral view 25 Frontal process (cocoon cutter), lateral view 25a Frontal process (cocoon cutter), ventral view 26 Two pairs of sensillae on head 27 Close-up of the paired sensillae on head 28 Setae on A2, dorsal-lateral view, arrows indicating the positions of setae 29 Segments A9−10, ventral view 30 Genital orifice 31 Lateral cremaster, lateral view 32 Ventral cremaster, ventral view.
The new species mostly resembles T. prosacta, especially in the male genitalia. However, it can be recognized by the male genitalia with the tegumen having 3−4 long setae on each lateral side, and the valva width does not change in the basal 3/4; in T. prosacta, the tegumen has 9−12 long setae on each lateral side and the valva is slightly wider at middle. In the female genitalia, T. grewiae sp. n. can easily be separated from all other Telamoptilia by the extremely short ductus bursae, not reaching the anterior margin of the seventh abdominal segment. The signum lacks a median process and is thereby more similar to signa in Spulerina, especially to that of S. parthenocissi Kumata & Kuroko, 1988, than to those in Telamoptilia.
Holotype, ♂, China: Mt. Baxian National Nature Reserves (117°33'N, 40°11'E), 300−600 m, Ji county, Tianjin, larva coll. 6-ix-2013, ex.Grewia biloba, emerged 9-iii-2014 (indoors), leg. Tengteng Liu. Paratypes: 2♂, 1♀, larvae coll. 24-vi-2013, emerged 6−7-vii-2013, 1♂, 1♀, larva coll. 29-vi-2013, emerged 5-vii-2013, 4♂, 3♀, larva coll. 8-viii-2013, emerged 19−20, 22-viii-2013, 1♂, 1♀, emerged 13, 22-ix-2013, 1♂, larva coll. 12-ix-2013, emerged iv-2014 (indoors), 2♀, larva coll. 30-vi-2014, other data as holotype, genitalia slide Nos. LTT12255♀, LTT12256−7♂, LTT12261♂, LTT12555♀, LTT12556−7♂; 1♂, 300 m, 29-vi-2014, by light, leg. Kaijian Teng & Tengteng Liu, other data as holotype, genitalia slide No. LTT12561.
5 larvae, 25-vi-2013, 6-ix-2013, stored in ethanol, other data as holotype, BXS130628, BXS130942; 1 last instar larval skin, larva coll. 6-ix-2013, emerged 13-iii-2014, other data as holotype, mounted in Canada balsam, slide no. LTT1403L; 1 last instar larval skin, 24-vi-2013, stored in glycerine, other data as holotype, BXS130632; 2 pupal exuviae, 29-vi-2014, other data as holotype, mounted in Canada balsam, slide Nos. LTT1401−2L.
Grewia biloba G. Don and its variety parviflora Hand. –Mazz. (Malvaceae). Telamoptilia grewiae sp. n. is thus far the only species in Gracillariinae that is known to feed on Grewia. The plant family Malvaceae appears to be the main host for Telamoptilia with now four out of six species feeding on this family (T. tiliae on Tilia, T. cathedraea on Urena, T. geyeri on Pavonia).
China (Tianjin).
The larva mines on the upper surface of the host plant leaf. The mine begins as an epidermal silvery curved white line which soon enlarges to a whitish blotch. Yellowish-fuscous or fuscous lines can be found on the surface of the blotch. As the larva develops, the blotch usually incorporates the earlier linear mine. The last instar larva vacates the mine for pupation by chewing a semicircular opening near the margin of the blotch. No body colour transfer occurs in the full-grown larva of this species, compared to other Telamoptilia larvae which turn red when fully grown (
The specific name is derived from the host plant genus Grewia, indicating the host of the new species.
The forewing venation of T. grewiae sp. n. is unique within the Acrocercops group: R1 and R5 are absent, thus R4 is not stalked with R5. The absence of R1 is apomorphic for Chrysocercops Kumata & Kuroko, 1988 (occasionally present), Telamoptilia and Spulerina (
Special thanks are indebted to the staff of the Baxian Mountain National Nature Reserves, for their persistent support during the field work; to the two reviewers and the editor Dr. Erik van Nieukerken for their insightful comments. This study was supported by the National Natural Science Foundation of China (No. 31272356) and the Research Fund for the Doctoral Program of Higher Education (No. 20130031110008).