Research Article |
Corresponding author: Eduardo Suarez-Morales ( esuarez@ecosur.mx ) Academic editor: Danielle Defaye
© 2014 Eduardo Suarez-Morales, Juan Fuentes.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Suarez-Morales E, Fuentes J (2014) A new species of Halicyclops (Copepoda, Cyclopoida, Cyclopidae) from a lagoon system of the Caribbean coast of Colombia. ZooKeys 459: 35-47. https://doi.org/10.3897/zookeys.459.7972
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Plankton samples obtained from the lagoon system Laguna Navío Quebrado, in northern Colombia, yielded male and female specimens of an undescribed cyclopoid copepod of the genus Halicyclops. The new species belongs to the highly diverse and widely distributed thermophilus-complex. It closely resembles H. clarkei Herbst, 1982 from Louisiana and H. bowmani Rocha & Iliffe, 1993 from Bermuda. These species share the same armature of P1-P4EXP3, with a 3443 spine formula and the terminal antennary segment with 5 setae. However, H. gaviriai sp. n. can be separated from both H. clarkei and H. bowmani by the morphology of the anal pseudoperculum, the proportions of the fourth antennulary segment, the length of the inner basipodal spine of P1, the P1EXP/inner basipodal spine inner length ratio and the length/width ratio of the caudal rami. This is the third species of Halicyclops recorded from Colombia and the first one described from this country. With the addition of H. gaviriai sp. n., the number of species of Halicyclops known from the Neotropics increases to 19. The regional diversity of the genus is probably underestimated.
Brackish waters, taxonomy, crustaceans, halicyclopines, lagoon systems biota
The cyclopoid copepod genus Halicyclops is the most speciose in the subfamily Halicyclopinae; currently, it is known to contain 111 species and subspecies (
In the Americas, Brazil and the United States are the countries with most records of Halicyclops (
Plankton samples were taken monthly from the Laguna Navío Quebrado, Colombia (11°25'N, 73°5'W) between April and December 2012, mainly in the littoral areas with vegetation (macrophytes and mangrove) but also from open water in areas close to oyster banks. Water salinity was measured with a WTW 3111 conductivity meter. Water samples were collected using a bucket of 25 L at both vegetation areas and shallow open water. Samples were filtered with a zooplankton net (45 μm) and preserved in 70% ethanol. Copepods were sorted from the original samples and then processed for taxonomical identification. Dissected specimens and appendages were mounted in glycerine and sealed with Canada balsam. Drawings were made with the aid of a camera lucida mounted on an Olympus BX51 compound microscope equipped with Nomarski DIC. The specimens were measured in lateral position, from the anterior end of the rostral area to the posterior margin of the caudal ramus. The specimens examined were deposited at the Museo de Colecciones Biológicas at the Universidad del Atlántico (UARC), Colombia and in the Collection of Zooplankton (ECO-CHZ) held at El Colegio de la Frontera Sur (ECOSUR), Chetumal, Mexico. Morphological terminology follows
Adult female holotype (UARC393Z), Laguna Navío Quebrado, Colombia, limnetic plankton sample, 7 N., 2007, coll. Juan M. Fuentes-Reinés. Male allotype (UARC394Z), both partially dissected. Paratypes: ten females and four males, undissected, ethanol-preserved, vial (UARC395Z), plus one dissected female, slides (UARC399Z-403Z) and one dissected male (UARC397Z). Three adult females from same locality and date, two of them undissected, ethanol-preserved, in vial, one mounted on slide (ECO-CHZ-09267).
Laguna Navío Quebrado, La Guajira, northern Colombia (11°25'N; 73°5'W).
Habitus in dorsal position as in Figure
Cephalosome with large rounded dorsal integumental window. Urosome with four segments, genital double somite as long as wide with slight lateral protrusion at halflength and rounded integumental window on each side of posterior half (Fig.
Antennules 6-segmented, setal formula as follows, s=setae, ae=aesthetasc: 1(8s), 2(12s), 3(3s), 4(5s), 5(3+ae), 6(10+ae); fourth segment about 1.7 times as long as wide (Fig.
Antenna consisting of 4 segments, coxa reduced and unarmed, basis with 2 setae at inner corner; seta representing EXP present. ENP two-segmented. Proximal endopodal segment with a seta on middle inner margin. Terminal endopodal segment about 1.4 times as long as preceding segment armed with 5 inner setae and 7 apical setae plus short spinule on proximal outer margin. Length/wide ratio of second segment about 2.3 (Fig.
Mandible with well-developed coxal gnathobase, armed with 7 teeth plus outermost dorsal pinnate seta. Palp reduced, represented by 2 naked setae inserted on small protuberance, one seta about 1/3 times as long as the other one (Fig.
Maxillule with praecoxal arthrite bearing four strong tooth-like spines distally, inner spine strongest, with two proximal subequal setae, inner surface with two robust setal elements and one regular seta. Palp two-segmented, basis with 4 setae, endopodite represented by single oval-shaped segment, armed with three subequal, lightly setulated setae (Fig.
Maxilla 4-segmented, comprising praecoxa, coxa, basis and 1-segmented endopod. Praecoxal endite robust, armed with 3 setae and 2 spiniform elements on inner margin, with distal set of four robust claw-like spines. Basis with three elements including a claw-like spine, one naked stout seta and a short slender seta, exopod represented by single proximal seta. Endopod with 3 setae (Fig.
Maxilliped 2-segmented, armed with 3 setal elements on basal segment and 5 setae on distal segment, one of them subdistal, two distal (Fig.
P1-P4 exopod and endopod 3-segmented (Fig.
P5 exopod subrectangular (Fig.
Habitus resembling that of female, body length, excluding caudal setae= 420µm; (average = 410 μm; n = 10; holotype: 420 μm). Cephalosome with middle integumental window dorsally and lateral window on posterior margin. Second and third somites of prosome with integumental windows laterally, the latter being smallest (Fig.
P1-P4 as in female (Fig.
The species is named after Dr. Santiago Gaviria for his work on Colombian copepods and his leadership in the formation of new generations of planktologists.
Halicyclops gaviriai sp. n. is assigned to the group of species “B” of Halicyclops with a 3443 spine formula; this is the most diverse group containing 74 species (see
Among the species of Halicyclops reported from the Caribbean region and adjacent areas (
The length/width ratio of the fourth antennulary segment differs in these species, it is much shorter in H. gaviriai (ratio = 1.7) vs. 2.5 in H. bowmani (
The armature details of P4ENP3 shows some additional differences among these species; this segment is armed with 4 spines and one spiniform, distally serrate seta in both H. clarkei (
The new species has also affinities with Halicyclops cf. clarkei from Panama (
The male of the new species H. gaviriai differs from the male of H. clarkei in the presence of modified setae on the antennular segments 10-11, lacking in H. clarkei (
Halicyclops gaviriai sp. n. is characterized by a unique combination of characters including: 1) last antennary segment with five lateral setae, 2) length/wide ratio of same segment over than twice as long as wide, 3) fourth segment of female antennule over than twice as long as wide, 4) inner basipodal spine of P1 reaching the posterior border of the ENP2 of P1, 5) ENP3 of P4 with four serrate spines and one seta, and 6) P5 about 1.45 times as long as wide, apical seta longer than the segment.
Distribution and ecology. Halicyclops gaviriai sp. n. is currently known from a single locality only, the protected coastal system Laguna Navío Quebrado, on the Caribbean coast of Colombia. This species was recorded in both the limnetic region and the vegetation zones, being more frequent in the former habitat. This large (surface area of 10.7 km2) lagoon system is a shallow water body (depth 0.3–1.1 m), whose temperature varies over the seasons in the range of 28–31 °C; pH values during sampling ranged between 7.8 and 8.3 and salinity was 28 PSU. This habitat diverges from that of one of its closest congeners, H. bowmani, a stygobitic form recorded only from an anchialine system of Bermuda (
The number of Neotropical species recognized by
Halicyclops gaviriai sp. n., adult holotype female from northern Colombia. A urosome showing genital somite, ventral view B anal somite showing anal pseudoperculum, dorsal view C internal structures of genitalia, ventral view, D proximal section of middle apical setae of caudal ramus E leg 5; adult male from same locality F urosome, ventral view G lateral view of cephalothorax showing position of integumental windows H geniculate antennule, showing brush-like modified setae on segments 10–12. Scale bars: A,B, F, H =50 μm, C–E= 25 μm, G=100 μm.