Short Communication |
Corresponding author: Peter Kaňuch ( kanuch@netopiere.sk ) Academic editor: Fernando Montealegre-Z
© 2017 Peter Kaňuch, Martina Dorková, Andrey P. Mikhailenko, Oleg A. Polumordvinov, Benjamín Jarčuška, Anton Krištín.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Kaňuch P, Dorková M, Mikhailenko AP, Polumordvinov OA, Jarčuška B, Krištín A (2017) Isolated populations of the bush-cricket Pholidoptera frivaldszkyi (Orthoptera, Tettigoniidae) in Russia suggest a disjunct area of the species distribution. ZooKeys 665: 85-92. https://doi.org/10.3897/zookeys.665.12339
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Phylogenetic analysis and assessment of the species status of mostly isolated populations of Pholidoptera frivaldszkyi in south-western Russia occurring far beyond the accepted area of the species distribution in the Carpathian-Balkan region were performed. Using the mitochondrial DNA cytochrome c oxidase subunit I gene fragment, we found a very low level of genetic diversity in these populations. Phylogeographic reconstruction did not support recent introduction events but rather historical range fragmentation. The grouping of the Russian and Romanian haplotypes in a distinct phylogenetic clade suggests that the pre-glacial range of P. frivaldszkyi had extended towards the Ponto-Caspian region, with considerable gene flow between different refugia. However, post-glacial northward expansion of the species from supposed Caucasus refugia contributed most likely to the current disjunct distribution of this relict-like bush-cricket.
Declining species, Insecta , mountain habitats, mtDNA, phylogeography, range fragmentation
The Green dark bush-cricket, Pholidoptera frivaldszkyi (Herman, 1871), is a regionally rare and endangered grassland-dwelling Orthoptera (Tettigoniidae) species with a decreasing population trend (
A total of 26 adult individuals of P. frivaldszkyi (12 males and 14 females) were collected at seven sites (3–6 ind. per site) from the Kursk (sites Petropavlovka, Bogatyrevo, Panino), Tambov (Novospasskoe, Khobotovo, Ranino) and Penza (Krutec) regions in Russia (51.5367°–53.0925°N, 36.0906°–44.5840°E, 140–230 m a.s.l., Fig.
Sampled sites of Pholidoptera frivaldszkyi populations in Central and Eastern Europe. Three geographically homogeneous genetic clusters in the Carpathian Mountains defined by spatial analysis of molecular variance are colour coded according to
A very low level of genetic diversity was found within seven mostly isolated populations of P. frivaldszkyi in Russia. Analysis of the 778 bp long sequences of mtDNA COI gene in 26 individuals revealed only two (0.3%) variable sites with three unique haplotypes (GenBank accession numbers KY554960–KY554962), which may indicate a strong demographic decline (cf.
Maximum-likelihood (ML) phylogenetic tree for 16 haplotypes of Pholidoptera frivaldszkyi (pf1–pf16; GenBank accession numbers KF706416–KF706428, KY554960–KY554962) with outgroup species (KC852400, KY554963–KY554966) based on a 778 bp fragment of the mtDNA COI gene. Tree topology and branch lengths of Bayesian inference were congruent with ML analysis. Nodes with significant support values are indicated (upper, ML bootstrap > 50%; lower, Bayesian posterior probability > 0.90).
The well preserved mountain meadows in the Carpathian-Balkan region (average altitude 650 m a.s.l.) are considered as typical habitat for P. frivaldszkyi (e.g.
The most plausible evolutionary explanation of the observed phylogeographic pattern is range contraction during the last glacial period and subsequent post-glacial species expansion. Traces of a genetic connection between the eastern Romanian and Russian populations suggest that the pre-glacial range of P. frivaldszkyi also extended towards the Ponto-Caspian region, similarly to the ranges of many other recent Orthoptera species (e.g.
We are grateful to Sandra Viglášová for her lab assistance and to Sigfrid Ingrisch and Klaus-Gerhard Heller for their comments on the manuscript. This study was supported by the Slovak Scientific Grant Agency VEGA (grants no. 2/0061/15, 2/0097/16).