Research Article |
Corresponding author: Giacomo Bernardi ( bernardi@ucsc.edu ) Academic editor: Maria Elina Bichuette
© 2017 Giacomo Bernardi, Gary C. Longo, T.E. Angela L. Quiros.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Bernardi G, Longo GC, Quiros TEAL (2017) Altrichthys alelia, a new brooding damselfish (Teleostei, Perciformes, Pomacentridae) from Busuanga Island, Philippines. ZooKeys 675: 45-55. https://doi.org/10.3897/zookeys.675.12061
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A new species of damselfish, Altrichthys alelia sp. n. is described from specimens collected in shallow water (1–8m depth) off Busuanga Island, Palawan Province, Philippines. It differs from the other two species in the genus, A. curatus and A. azurelineatus, in various features including having golden upper body lacking dark edges of dorsal and caudal fins, higher modal number of tubed lateral line scales, as well as differences in two mitochondrial markers, one nuclear marker, and RAD markers.
Apelagic fishes, Acanthochromis , CO1, Control region, RAD markers
The damselfish genus Altrichthys Allen, 1999 includes two species, the azure damselfish, Altrichthys azurelineatus (Fowler & Bean, 1928), and the guardian damselfish, Altrichthys curatus Allen, 1999, that occur on shallow coral reefs in the Calamian Archipelago, northern Palawan Province, Philippines (
Specimens were collected with hand nets while free or scuba diving in less than 8m depth. Counts and measurements follow
Fin clip tissue samples were stored in 95% ethanol and DNA was extracted using DNeasy Blood & Tissue kits (Qiagen) according to the manufacturer’s protocol. DNA was extracted from two Altrichthys alelia individuals (juvenile individuals 6 and 8, in
Type locality of Altrichthys alelia. Busuanga Island, Palawan Province, Philippines, near the village of Maricaban, next to the resort “El Rio y Mar”, 12.1911°N; 120.1022°E. Highlighted in red are Tara Island and Uson Island, where specimens of Altrichthys were first collected, as well as Uson Island and Sangat Island where sister species A. azurelineatus and A. curatus were collected.
In addition, we constructed RAD libraries using a variation of the original protocol with restriction enzyme SbfI (
San José, Busuanga Island, Philippines, 12.1911°N ; 120.1022°E.
Altrichthys azurelineatus. Holotype: USNM 89957 (one specimen, Uson Island), Paratypes USNM 96398 (one specimen Tara island), USNM 96425 (one specimen, Tara Island). Altrichthys azurelineatus 5 specimens from Uson Island, A. curatus 5 specimens from Uson Island and Sangat Island.
A species of Altrichthys distinguished by the following combination of characters: dorsal rays XIV, 13–14; anal rays II, 15, tubed lateral line scales 14–15 (Table
Morphometric and Meristic measurements of Altrichthys. Lines present, from top to bottom, numbers for Total Length (TL), Standard Length (SL), Depth, With, Eye Diameter (Eye), spine and ray counts for Dorsal and Anal fins, and Tubed Lateral Line Scales (Tubed LL scales). Number in parenthesis represent percentage of the Standard length). The first column is the species holotype.
Voucher number |
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9 | 10 | 11 | 12 | |
TL (mm) | 73.6 | 66.8 | 66.2 | 70.2 |
SL (mm) | 54.9 | 51.6 | 51.0 | 54.1 |
Depth (mm) | 31.6 (57.6%) | 30.2 (58.5%) | 30.0 (58.8%) | 31.2 (57.7%) |
Width (mm) | 9.6 (17.5%) | 11.0 (21.3%) | 11.6 (22.7%) | 11.1 (20.5%) |
Eye (mm) | 5.2 (9.5%) | 5.4 (10.5%) | 5.6 (11.0%) | 5.7 (10.5%) |
Dorsal fin | XIII, 14 | XIV, 13 | XIV, 13 | XIII, 14 |
Anal fin | II,15 | II,15 | II,15 | II,15 |
Tubed LL scales | 15 | 14 | 14 | 15 |
In addition, Sanger sequencing results show that Altrichthys alelia individuals form a group most closely related to, but distinct from, A. azurelineatus (Figure
Phylogenetic reconstructions of Altrichthys species based on mitochondrial control region (313 aligned base pairs) and cytochrome oxydase 1 (CO1, 611 aligned base pairs). The closely related Acanthochromis polyacanthus was used as an outgroup. Sample labels refer to collection sites: BUS, El Rio y Mar Resort, Busuanga Island, COR, Balinsasayaw Resort, Uson Island; SAN, Sangat Island; DLR, Dive Link Resort, Uson Island. Numbers above nodes refer to percent of 1000 bootstrap replicates used in Neighbor-Joining (left) and Maximum Likelihood (right) reconstruction methods.
Results of RAD sequencing in three species. Results are based on 8383 variable SNPs for 5 sequenced individuals (2 A. alelia, 1 A. azurelineatus, 2 A. curatus). Figures refer to the number of SNPs and their percentage (over 8383 total SNPs).
A. alelia | A. azurelineatus | A. curatus | |
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A. alelia | 123 / 1.47% | ||
A. azurelineatus | 1584.5 / 18.9% | – / – | |
A. curatus | 7224.5 / 86.2% | 6495 / 76.4% | 8 / 0.0% |
Known from northern Busuanga Island at San José, Palawan Province, Philippines (Figure
Collected off live and extensive thickets of corals mostly Porites cylindrica.
The name Altrichthys alelia derives from the combined first names of Alessio Bernardi and Amalia Bernardi, who greatly helped during field-work on Altrichthys.
We suggest Alelia’s damselfish as a literal translation of the scientific name.
Species of the genus Altrichthys together with their close relative Acanthochromis polyacanthus are unusual as they brood their young (
Altrichthys alelia may have been observed previously by other scientists, but remained unnoticed. The original description of Chromis azurelineatus (Fowler & Bean, 1928), later re-described as Altrichthys azurelineatus (Allen, 1999), was based on fish collected during the Albatross expedition of 1908-1909. Three collections were made in December 1908 (
Drawing (upper left, from
We would like to thank Nicole Crane, Amalia Bernardi, and Alessio Bernardi for help in the field. We thank the following: Bureau of Fisheries and Aquatic Resources (Commodity Clearance 2016-20091), Palawan Council for Sustainable Development (PCSD, GP 2016-03, Wildlife Transport Permit No. 2016-05-000062-DMO – Calamianes, Fisheries inspection and quarantine clearance Permit no. 009391) and Leonora Labrador and Eva Palanca of the Coron PCSD office. Ateneo de Manila University’s Hendrik Freitag, Merab A. Chan, Jonathan Patrick H. Yan for the Memorandum of Agreement between UCSC and the Department of Biology at Ateneo de Manila. Busuanga Mayor (Samuel de Jesus) and Councilor (Ann D. Mercado), Coron Mayor (Clara Espiritu-Reyes) and Samuel Madamba, Culion Mayor (Emiliano Marasigan) and Councilor Leonilo Sarmiento for Prior Informed Consent (PIC), given in 2015. Hikari South Sea Pearl, Luna Sombrano, Agustin R. Badon II, Eric T. Dalisay, and Pinky Perpetua for assistance in obtaining the PICs. We thank El Rio y Mar Resort, Dive Link Resort, and Balinsasayaw for letting us visit their reefs, and Gerald Allen for discussing our data and invaluable information on Altrichthys. We are very grateful to Sandra J. Raredon for taking pictures of Altrichthys specimens at the Smithsonian collection, and to Don Dumale (National Museum of the Philippines) and David Catania (California Academy of Science) for providing Holotype and Paratype numbers. This work was funded by a National Geographic Grant and a UCSC Committee on Research (COR) grant to GB.