Research Article |
Corresponding author: Yuri M. Marusik ( yurmar@mail.ru ) Academic editor: Shuqiang Li
© 2016 Xiaoqing Zhang, Yuri M. Marusik.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang X, Marusik YM (2016) A survey of Pireneitega from Tajikistan (Agelenidae, Coelotinae). ZooKeys 635: 89-107. https://doi.org/10.3897/zookeys.635.10487
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Five new species of Pireneitega species from Tajikistan are described: P. zonsteini sp. n. (♂♀), P. muratovi sp. n. (♀), P. tyurai sp. n. (♀), P. ramitensis sp. n. (♀) and P. kovblyuki sp. n. (♂). Pireneitega major (Kroneberg, 1875) is redescribed for the first time based on the lectotype designated here. DNA barcodes for the five new species are documented for future use and as proof of molecular differences between these species.
Aranei , central Asia, description, new species, Paracoelotes, redescription, spider, taxonomy
Coelotinae is the largest subfamily of Agelenidae with more than 650 species distributed in the Holarctic and southeast Asia (
Specimens were examined and measured with a Leica M205C stereomicroscope. Images were captured with an Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on an Olympus SZX12 dissecting microscope. Epigynes and male palps were examined after dissection. Epigynes were cleared by boiling it in 10% KOH solution before taking photos of the dorsal view. All measurements are given in millimeters. Pireneitega major was photographed and drawn using an MBS-9 stereomicroscope with Pro-MicroScancamera. Leg measurements are given as: total length (femur, patella + tibia, metatarsus, tarsus).
Terminology used for copulatory organ characters in the text and figure legends follows
Abbreviations used in the text and figure legends are:
A epigynal atrium
ALE anterior lateral eye
AME anterior median eye
AME-ALE distance between AME and ALE
AME-AME distance between AME and AME
ALE-PLE distance between ALE and PLE
CD copulatory ducts
CF cymbial furrow
CO conductor
d dorsal
E embolus
EB embolic base
ET epigynal teeth
FD fertilization ducts
Fe femur
H epigynal hood
MA median apophysis
Mt metatarsus
p prolateral
PA patellar apophysis
Pa patella
PLE posterior lateral eye
PME posterior median eye
PME-PLE distance between PME and PLE
PME-PME distance between PME and PME
R receptacle
r retrolateral
RTA retrolateral tibial apophysis
ST subtegulum
T tegulum
Ta tarsus
Ti tibia
v ventral
VTA ventral tibial apophysis
DNA barcodes were obtained for future use: a partial fragment of the mitochondrial gene cytochrome oxidase subunit I (COI) was amplified and sequenced for five new species using Primers LCO1490-oono (5’-CWACAAAYCATARRGATATTGG-3’) (
Species | GenBank accession number | Sequence length | Collection localities |
---|---|---|---|
P. zonsteini sp. n. | KY024475 | 642bp | Env. of Dushanbe, Hissar Mt. Ridge 48th km of Varzob Hwy |
P. muratovi sp. n. | KY024477 | 642bp | Env. of Dushanbe Hissar, Mt. Ridge 20th km of Varzob Hwy Gusgarf Vill. |
P. tyurai sp. n. | KY024478 | 642bp | Khatlon Area Khovaling Distr., Obimazar River |
P. ramitensis sp. n. | KY024476 | 642bp | Khatlon Area Hissar Mt. Range Ramit Reserve |
P. kovblyuki sp. n. | KY024474 | 642bp | Tajikstan: Khatlon Area Dangara Distr Sanglogh |
Holotypes and some paratypes will be deposited in the
Pireneitega Kishida, 1955: 11. Type species Amaurobius roscidus L. Koch, 1868 (= P. segestriformis (Dufour, 1820)) from Germany.
Paracoelotes Brignoli, 1982: 347. Type species Coelotes armeniacus Brignoli, 1978 from Turkey.
Pireneitega was long considered a nomen nudum (Yaginuma, in
The chelicerae in most species of Pireneitega (including the type species) have 3 promarginal and 3 retromarginal teeth; other coelotines have either 2 or 4 retromarginal teeth (
Thirty species of Pireneitega are known from Spain to Sakhalin (
Holotype ♂ (
The species is named after Sergei Zonstein (University of Tel-Aviv, Israel) a partner of the junior author in the expedition to Tajikistan; noun (name) in genitive case.
The male can be distinguished from all other Pireneitega species except P. involuta (
Male (holotype): Total length 8.90. Carapace 4.40 long, 3.50 wide. Abdomen 4.50 long, 2.80 wide. Eye sizes and interdistances: AME 0.15, ALE 0.20, PME 0.15, PLE 0.20; AME-AME 0.07, AME-ALE 0.06, PME-PME 0.15, PME-PLE 0.18. Leg measurements: I: 12.95 (3.50, 4.30, 3.15, 2.00); II: 12.25 (3.25, 4.00, 3.00, 2.00); III: 10.40 (3.15, 3.00, 3.25, 1.00); IV: 16.00 (4.50, 5.00, 4.25, 2.25). Carapace greenish, the radial grooves indistinct, with black lateral margins. Abdomen blackish, with yellow herringbone pattern.
Spination in male
Fe | Pt | Ti | Mt | Ta | |
---|---|---|---|---|---|
I | 3d 2p 1r | – | 3-3v | 3-3v | – |
II | 3d 1p 1r | – | 2p 3-3v | 2p 3-3v | – |
III | 3d 2p 2r | 1p 1r | 1d 2p 2r 3-3v | 2p 2r 3-3v | – |
IV | 3d 2p 1r | 1p 1r | 2p 2r 3-3v | 2p 2r 3-3v | – |
Palp as in Fig.
Female (paratype): Total length 10.0. Carapace 4.75 long, 3.65 wide. Abdomen 5.25 long, 3.45 wide. Eye sizes and interdistances: AME 0.20, ALE 0.25, PME 0.21, PLE 0.26; AME-AME 0.08, AME-ALE 0.05, PME-PME 0.17, PME-PLE 0.20. Leg measurements: I: 12.50 (3.75, 4.25, 2.75, 1.75); II: 11.75 (3.50, 4.00, 2.75, 1.50); III: 10.60 (3.00, 3.50, 2.60, 1.50); IV: 15.00 (4.25, 4.75, 4.00, 2.00). Carapace yellow. Abdomen black, with yellow spots and herringbone pattern.
Epigyne as in Fig.
Spination in female
Known only from the type locality (Fig.
Holotype ♀ (
The species is named after Tajikistan zoologist Rustam Muratov (Dushanbe, Tajikistan) who was very helpful in organizing the expedition to Tajikistan; noun (name) in genitive case.
The female can be distinguished from all other Pireneitega species except P. fedotovi, P. luniformis (Zhu & Wang, 1994), and P. major by having narrow epigynal teeth and an elongate oval atrium. It can be distinguished from P. fedotovi by the pointed tip of septum (vs blunt tip in P. fedotovi), from P. luniformis by the elongate oval receptacles (vs spiralled in P. luniformis), and from P. major by its short epigynal teeth, ca. 0.8 times as long as length of the atrium (vs long epigynal teeth in P. major, about as long as the length of the atrium) (Figs
Male: unknown.
Female (holotype): Total length 9.94. Carapace 4.49 long, 3.05 wide. Abdomen 5.45 long, 2.90 wide. Eye sizes and interdistances: AME 0.18, ALE 0.23, PME 0.24, PLE 0.30; AME-AME 0.10, AME-ALE 0.05, PME-PME 0.15, PME-PLE 0.10. Leg measurements: I: 11.25 (3.25, 4.00, 2.50, 1.50); II: 10.30 (3.00, 3.50, 2.50, 1.30); III: 9.70 (2.75, 3.00, 2.65, 1.30); IV: 13.75 (3.75, 4.25, 4.00, 1.75). Carapace yellow, the radial grooves indistinct. Abdomen whitish-yellow, with green herringbone pattern.
Epigyne as in Fig.
Spination
Known only from the type locality (Fig.
Holotype ♀ (
The species is named after Sergei V. Tyura (Magadan, Russia) a friend of the junior author; noun (name) in genitive case.
The female can be distinguished from all other Pireneitega species except P. tianchiensis (
Male: unknown.
Female (holotype): Total length 5.15. Carapace 2.15 long, 1.75 wide. Abdomen 3.00 long, 2.00 wide. Eye sizes and interdistances: AME 0.10, ALE 0.13, PME 0.15, PLE 0.15; AME-AME 0.05, AME-ALE 0.10, PME-PME 0.02, PME-PLE 0.04. Leg measurements: I: 6.20 (1.90, 2.25, 1.25, 0.80); II: 5.10 (1.60, 1.75, 1.00, 0.75); III: 4.80 (1.50, 1.60, 1.00, 0.70); IV: 7.05 (2.05, 2.50, 1.50, 1.00). Carapace yellow, with black lateral margins. Abdomen blackish, with yellow herringbone pattern.
Epigyne as in Fig.
Spination
Known only from the type locality (Fig.
Holotype ♀ (
The specific name is an adjective and refers to the type locality; adjective.
The female can be distinguished from all other Pireneitega species except P. muratovi sp. n., P. fedotovi, P. luniformis and P. major, by having an elongate oval atrium, narrow epigynal teeth, and long copulatory ducts. It can be distinguished from P. muratovi sp. n. and P. luniformis by the narrow tip of the copulatory ducts (vs round tip in P. muratovi sp. n. and P. luniformis) and from P. fedotovi and P. major by the bent epigynal teeth (vs straight epigynal teeth in P. fedotovi and P. major) (Figs
Male: unknown.
Female (holotype): Total length 12.00. Carapace 4.50 long, 3.55 wide. Abdomen 7.50 long, 4.75 wide. Eye sizes and interdistances: AME 0.20, ALE 0.23, PME 0.25, PLE 0.20; AME-AME 0.10, AME-ALE 0.20, PME-PME 0.10, PME-PLE 0.23. Leg measurements: I: 14.05 (4.00, 4.75, 3.45, 1.85); II: 13.40 (3.90, 4.50, 3.25, 1.75); III: 13.00 (3.75, 4.25, 3.25, 1.75); IV: 16.55 (4.75, 5.40, 4.40, 2.00). Carapace yellowish, with brown lateral margins. Abdomen pale-yellow, with brown spots.
Epigyne as in Fig.
Spination
Known only from the type locality (Fig.
Holotype ♂ (
The specific name is a patronym in honour of the well known arachnologist and friend of the junior author Mykola M. Kovblyuk (Simferopol, Ukraine); noun (name) in genitive case.
The male can be distinguished from all other Pireneitega species except P. tianchiensis by having a hook-shaped conductor, and narrow cymbium. It can be distinguished from P. tianchiensis by the short cymbial furrow, ca. 1/10 length of cymbium (vs long cymbial furrow in P. tianchiensis, about 0.5 length of cymbium) (Fig.
Male (holotype): Total length 7.90. Carapace 4.00 long, 3.00 wide. Abdomen 3.90 long, 2.65 wide. Eye sizes and interdistances: AME 0.15, ALE 0.20, PME 0.18, PLE 0.19; AME-AME 0.08, AME-ALE 0.07, PME-PME 0.13, PME-PLE 0.15. Leg measurements: I: 10.90 (3.25, 4.05, 2.00, 1.60); II: 9.85 (3.00, 3.50, 2.00, 1.35); III: 8.60 (2.75, 2.50, 2.10, 1.25); IV: 12.55 (3.70, 3.75, 3.50, 1.60). Carapace yellow, the radial grooves indistinct. Abdomen pale, with yellow herringbone pattern.
Palp as in Fig.
Spination
Fe | Pt | Ti | Mt | Ta | |
---|---|---|---|---|---|
I | 3d 2p 1r | – | 3-3v | 3-3v | – |
II | 3d 3p 1r | 1p | 2p 3-3v | 3p 3-3v | – |
III | 3d 2p 2r | 1d1p 1r | 1d 2p 2r 3-3v | 5p 5r 3-3v | 1p 1r |
IV | 3d 1p 1r | 1p 1r | 2p 2r 3-3v | 5p 5r 3-3v | 2p 1r |
Female: Unknown.
Known only from the type locality (Fig.
Coelotes major Kroneberg, 1875: 15, pl. 1, fig. 6 (♀); Charitonov, 1946: 20, fig. 5 (♀).
Paracoelotes major : Ovtchinnikov, 1988: 142 (transferred from Coelotes).
Misidentifications:
Coelotes
major
:
Paracoelotes
major
:
Lectotype ♀ (
The figures of P. major presented by
This species is easily distinguished from other species of Pireneitega found in Tajikistan by its larger size (carapace length >6 mm vs <4.75) and having 5 spines on tarsus IV (vs other species with 0‒4). The epigyne of P. major is most similar to that of P. muratovi sp. n. and P. ramitensis sp. n. It can be distinguished from P. muratovi sp. n. by its shorter receptacles with length/width ratio of 2.3 (vs 2.6 in P. muratovi), shape of copulatory ducts, and shorter teeth (cf. Figs
Male: unknown.
Female: Lectotype. Total length 16.7. Carapace 7.0 long, 5.0 wide, fovea 1.25 long. Leg measurements: I:19.75 (5.5, 2.5, 4.6, 4.65, 2.5); II: 18.6 (5.1, 2.5, 4.0, 4.5, 2.5); III: 17.2 (4.75, 2.2, 3.55, 4.6, 2.1); IV: 21.85 (5.75, 2.3, 5.0, 6.25, 2.55).
Spination
Fe | Pt | Ti | Mt | Ta | |
---|---|---|---|---|---|
I | 3d 2p 2r | – | 3-3v | 3-3v 1vm | – |
II | 3d 3p 2r | – | 2p 3-3v | 1p 3-3v | – |
III | 3d 3p 2r | 1p 1(0)r | 2p 2r 3-3v | 5p 4r 3-3v | 2p 1-1v |
IV | 3d 2p 1r | 1p 1r | 2p 2r 3-3v | 5p 4r 3-3v | 2p 3r |
Paralectotype ♀. Total length: 11.0. Carapace 6.0 long, 4.0 wide. Epigyne 0.51 wide.
Epigyne as in Fig.
Known from the type series females only. Exact locality is known for the lectotype only: Auchi lies on the northern macroslope of the Turkestan Mt Range (Fig.
We thank Sergei Zonstein (Tel-Aviv, Israel; a partner of the junior author in the expedition to Tajikistan), Rustam Muratov and Murod Saidov (Dushanbe, Tajikistan) for field assistance and helping to organize the expedition; Kirill G. Mikhailov (Moscow, Russia) for allowing us to study the type material of P. major; Seppo Koponen (Turku, Finland) for providing museum facilities in Turku; and Zhe Zhao (Beijing, China) for helping to provide the molecular data. English of the final draft was kindly checked by Robert Bennett (Victoria, British Columbia, Canada). This study was supported by the National Natural Sciences Foundation of China to Guo Zheng (NSFC-31172121,31372224,31672315).