Corresponding author: Eduard Solà (
Academic editor: F. Govedich
The taxonomic status of
Solà E, Sluys R, Segev O, Blaustein L, Riutort M (2015) The taxonomic status of
The freshwater planarian fauna of Israel has been relatively well studied (
In the course of our studies on the evolution and diversification of the genus
New samples of
Israeli sampling localities from where
Code | Locality | Species | Sampling date | Site description | Coordinates |
---|---|---|---|---|---|
SHE | Ein Shefa |
|
06/25/2009 | Fast flowing man made spring channel |
|
BAN | Nahal Banias |
|
08/27/2009 | Fast flowing stream |
|
BET | Nahal Betzet |
|
09/01/2009 | Isolated temporary pools within dry stream |
|
TEO | Ein Te’o |
|
02/03/2010 | Shallow spring with moderate water flow |
|
ENU | Ein Nun |
|
02/03/2010 | Shallow spring with moderate water flow |
|
EHU | Einot Huga | Not |
05/09/2010 | Shallow spring - rather saline water ≤2000 mg Cl/l |
|
EOV | Ein Ovdat |
|
05/09/2010 | Partly connected with slowly flowing spring pools of a desert stream |
|
TZU | Ein Tzuba |
|
05/10/2010 | Shallow man-made spring pool |
|
SAT | Ein Sataf |
|
05/10/2010 | Small spring pool inside a man-made underground cave |
|
GED | Ein Gedi | 08/04/2010 | Small shallow spring pool - desert area |
|
|
DAN | Dan Springs | Not |
08/18/2010 | Shallow slowly flowing stream |
|
Total genomic DNA was extracted by using the commercial reagent DNAzol (Molecular Research Center Inc., Cincinnanti, OH), following the manufacturer’s instructions. A fragment of the cytochrome c oxidase subunit I (
Forward (F) and Reverse (R) primers used in the amplification and sequencing of the
Name | Direction | Sequence 5’−3’ | Annealing temperature (°C) | Source |
---|---|---|---|---|
BarT | F | ATGACDGCSCATGGTTTAATAATGAT | 43 |
|
COIEF3 | F | CCWCGTGCWAATAATTTRAG | 43 |
|
COIR | R | CCWGTYARMCCHCCWAYAGTAAA | 43 |
|
The number of
Details on the Israeli individuals sequenced for the present work.
Individual | Locality | Polymorphic | Haplogroup | Haplotype in Figure |
GenBank Acc. Number |
---|---|---|---|---|---|
D01TEO | Ein Te’o | No | A | 7 |
|
D01BAN | Nahal Banias | No | B | 2 |
|
D02BAN | Yes | − | − |
|
|
D03BAN | Yes | − | − |
|
|
D04BAN | No | B | 2 |
|
|
D02SHE | Ein Sheva | No | B | 3 |
|
D03SHE | No | B | 3 |
|
|
D04SHE | Yes | − | − |
|
|
D05SHE | Yes | − | − |
|
|
D06SHE | No | B | 3 |
|
|
D01BET | Nahal Betzet | No | B | 8 |
|
D02BET | No | B | 8 |
|
|
D03BET | No | B | 8 |
|
|
D04BET | No | B | 3 |
|
|
D05BET | No | B | 3 |
|
|
D01TZU | Ein Tzuba | No | B | 4 |
|
D02TZU | No | B | 4 |
|
|
D03TZU | No | B | 4 |
|
|
D07TZU | No | B | 4 |
|
|
D08TZU | No | B | 4 |
|
|
D09TZU | No | B | 4 |
|
|
D10TZU | No | B | 4 |
|
|
D04SAT | Ein Sataf | No | B | 5 |
|
D05SAT | No | B | 5 |
|
|
D06SAT | No | B | 5 |
|
|
D07SAT | No | B | 5 |
|
|
D11SAT | Yes | − | − |
|
|
D06EOV | Ein Ovdat | No | B | 1 |
|
D07EOV | No | B | 1 |
|
|
D09EOV | No | B | 6 |
|
|
D01ENU | Ein Nun | Yes | − | − |
|
D02ENU | Yes | − | − |
|
|
D05ENU | Yes | − | − |
|
|
D09ENU | Yes | − | − |
|
|
D16ENU | Yes | − | − |
|
Material examined (collections Naturalis Biodiversity Center, Leiden):
Out of the 32 localities that we visited in Israel, about one-third (11) yielded specimens of
Map of Israeli localities sampled for this study:
Unfortunately, preservation and histological problems eventually prevented us of carrying out detailed morphological analyses on the reproductive apparatus of Israeli
We were successful in obtaining
Most of the Israeli
Haplotype network of
Additionally, we compared the polymorphic sequences of the Israeli
The results of our molecular analyses suggest a wide distribution of
We have been unable to find any stable structural morphological difference between
Characteristic of
The situation that (1) in the field some populations may reproduce asexually and show a triploid set of 3n = 27 + 2−3 B chromosomes, (2) others reproduce sexually and show a complement of 2n = 18 gradually decreasing, metacentric chromosomes, and (3) sexualized, triploid specimens show hyperplasic ovaries and poorly developed testes is well-known for
In addition to the morphological and karyological similarities between nominal
This conclusion holds true for one of the two Turkish populations of presumed
Presumed
We thank Heather Bromley for fruitful discussion. We are indebted to Gema Blasco for support in the laboratory. E. Solà acknowledges support from Synthesys, the European Union-funded Integrated Activities grant (project grant: NL-TAF 5151). This research was also supported by BES-2009-022530 Grant from the Ministerio de Ciencia e Innovación (to E. Solà), and by grants CGL2008-00378 and CGL2011-23466 (to M. Riutort).
Supplementary Table
occurence
Localities in Israel from which no specimens of