Phylogenetic tree (Maximum Likelihood, 50% majority consensus) of selected 28S partial sequences of calcareous sponge species belonging to the order Leucosolenida, including Paragrantia waguensis and Grantia compressa (asterisks). Sequences were downloaded from the NCBI website (http://www.ncbi.nlm.nih.gov/). The accession numbers and sources for the sequences are listed in Table 1. Bootstrap support values are based on 100 replicates. The species were selected on the basis of a BLAST search using a 430 bps 28S partial sequence of Paragrantia waguensis (specimen RMNH Por. 9317), provided by the Naturalis Biodiversity Center DNA laboratory. Sequences were aligned, trimmed and analyzed using MEGA 6.06 for Mac (http://www.megasoftware.net/megamac.php).