The ML tree (ln L = −8756.30) for 2,901 bp of mitochondrial COI, tRNACys, tRNAMet, 12S rDNA, tRNAVal, 16S rDNA, and ND1 markers. Voucher numbers of the specimens of Orobdella masaakikuroiwai sp. n. are accompanied by the collection locality numbers (see Fig. 1). The numbers associated with the nodes represent the bootstrap values for ML (BS)/and Bayesian posterior probabilities (BPPs).