Map of Brazil indicating the geographic locations from which Brazilian-1 (green), Brazilian-2 (blue) and Brazilian-3 (yellow) morphotypes of Anastrepha fraterculus were collected. 1 Parnamirim, RN 2 Maceio, AL 3 Conceição de Almeida, BA 4 Parati, RJ 5 Ubatuba, SP 6 Caraguatatuba, SP 7 Ilhabela, SP 8 São Sebastião, SP (region of sympatry of Brazilian-2 and Brazilian-3 morphotypes, Selivon et al. 2004, Hernández-Ortiz et al. 2012) 9 Maresias, SP 10 Miracatu, SP 11 Morretes, SP 12 Porto Belo, SC 13 Criciúma, SC 14 Pelotas, RS 15 Bento Gonçalves, RS 16 Vacaria, RS (region of sympatry of Brazilian-1 and Brazilian-3 morphotypes, Selivon et al. 2004) 17 São Joaquim, SC 18 Caçador, SC 19 Botucatu, SP 20 Piracicaba, SP 21 São Paulo, SP 22 Santa Isabel and Jambeiro, SP (Paraíba valley - region of sympatry of the three morphotypes, Selivon et al. 2004) 23 Uberlandia, MG.