NJ tree based on the COI sequences of the mtDNA of Chironomus (Diptera: Chironomidae) species. The sequence of Lipiniella fujiprimus was used as the outgroup. Bootstrap values > 50% are shown on branches. Accession numbers and countries are provided beside the species names. Species flagged with an asterisk (*) are neotropical species. Brazilian Chironomus population: C. sp1BRA; C. sp2BRA; C. sp3BRA