Neighbour-Joining tree of the barcoding sequences of mosquito species listed for Neotropics, based on Tamura-Nei genetic distances. Terminal branches have been collapsed in order to save space (see original tree in Suppl. material 5 and 6). Branches in colors indicate non-monophyletic genera. Red clusters represent groups with problems to discriminate species. Names with green asterisk indicate non- monophyletic species. Bootstrap values above 60 (1,000 replicates) are given at the nodes.